1 X DMEM High Glucose, With L-glutamine, With Sodium Pyruvate, With Pyridoxine HCI

$24.00

This high-performance cell culture medium is a complete formulation of Dulbecco’s Modified Eagle’s Medium (DMEM) optimized for fast-growing and energy-demanding cells. With 4.5 g/L glucose, it provides enhanced energy support for cancer cells, stem cells, and other high-metabolism cell types. The inclusion of L-glutamine supports protein synthesis, while sodium pyruvate aids in cellular energy production and pyridoxine HCl (vitamin B6) ensures proper metabolic function.

This high-performance cell culture medium is a complete formulation of Dulbecco’s Modified Eagle’s Medium (DMEM) optimized for fast-growing and energy-demanding cells. With 4.5 g/L glucose, it provides enhanced energy support for cancer cells, stem cells, and other high-metabolism cell types. The inclusion of L-glutamine supports protein synthesis, while sodium pyruvate aids in cellular energy production and pyridoxine HCl (vitamin B6) ensures proper metabolic function.

DMEM (Dulbecco’s Modified Eagle’s Medium) – High Glucose

DMEM is a widely used nutrient-rich cell culture medium in biological and biomedical research. This high-glucose formulation contains 4.5 g/L glucose, compared to 1 g/L in low-glucose versions, making it ideal for energy-demanding cells such as cancer cells, stem cells, and rapidly proliferating cell types.

Key Features & Usage

  • Cell Culture: Supports the growth of various mammalian cells, including fibroblasts, HeLa cells, and primary cell lines.

  • Research Applications: Used in studies on cell proliferation, differentiation, metabolism, and response to experimental treatments.

  • Stem Cell Culture: Preferred for culturing induced pluripotent stem cells (iPSCs) and other stem cells due to its nutrient-dense composition.

Supplementation & Handling

  • Fetal Bovine Serum (FBS): Typically added at 5–10% to provide essential growth factors, hormones, and nutrients.

  • Additional Supplements: Depending on cell line requirements, may include:

    • Antibiotics (e.g., penicillin-streptomycin)

    • Non-essential amino acids (NEAA)

    • Specialized growth factors

  • L-Glutamine Note: If the medium contains L-glutamine, monitor its stability and replenish as needed, as it degrades over time.

  • Sterile Technique: Essential during preparation and handling to prevent contamination.

Storage & Stability

  • Store at 2–8°C to maintain medium integrity.

  • Regularly check expiration dates and supplement stability (especially for labile components like L-glutamine).