1 X DMEM Low Glucose, With L-glutamine, With Sodium Pyruvate, With Pyridoxine HCI (1x500ML)

$16.00

Ideal for cell types that thrive in lower glucose environments, this DMEM Low Glucose formulation (1.0 g/L) provides balanced nutrition while minimizing metabolic stress. Enriched with L-glutamine for protein synthesis, sodium pyruvate for energy metabolism, and pyridoxine HCl (vitamin B6) for enzymatic cofactor support, this medium ensures optimal growth conditions for sensitive and specialized cell cultures.

Ideal for cell types that thrive in lower glucose environments, this DMEM Low Glucose formulation (1.0 g/L) provides balanced nutrition while minimizing metabolic stress. Enriched with L-glutamine for protein synthesis, sodium pyruvate for energy metabolism, and pyridoxine HCl (vitamin B6) for enzymatic cofactor support, this medium ensures optimal growth conditions for sensitive and specialized cell cultures.

Product Note: DMEM Low Glucose (1.0 g/L) with L-Glutamine, Sodium Pyruvate & Pyridoxine HCl

Key Considerations for Use:

  • L-Glutamine Stability: This formulation contains L-glutamine, which may degrade over time. We recommend:
    ✓ Regularly checking the expiration date
    ✓ Monitoring medium performance
    ✓ Supplementing with fresh L-glutamine if extended culture periods are required

  • Sterile Technique:
    ✓ Always use aseptic techniques during preparation and handling
    ✓ Work in a laminar flow hood when possible
    ✓ Filter supplements if added post-thaw

  • Standard Supplementation:
    ✓ Typically supplemented with 5-10% FBS (fetal bovine serum) for essential growth factors and nutrients
    ✓ May require additional components depending on cell type:
    • Antibiotics (e.g., penicillin/streptomycin)
    • Non-essential amino acids
    • Cell-specific growth factors

Product Overview:

DMEM Low Glucose (1.0 g/L) is a versatile basal medium optimized for:
✓ Primary cell cultures (neurons, hepatocytes, etc.)
✓ Glucose-sensitive applications (metabolic studies, differentiation protocols)
✓ Research requiring controlled glucose conditions (diabetes studies, signaling pathway analysis)

Storage & Handling Protocol:

  • Temperature: Maintain at 2-8°C (do not freeze)

  • Light Protection: Store in original packaging

  • Shelf Life: Use before expiration date on label

  • Thawing: If frozen supplements are added, warm gradually to 37°C

Experimental Flexibility:

This formulation serves as an excellent base medium that can be customized with various supplements to meet specific research requirements, making it invaluable for:
• Metabolic studies
• Stem cell differentiation
• Specialized cell culture applications