DMEM 1X, High Glucose, With L-Glutamine, Without Sodium Pyruvate, Without HEPES 

$24.00

Our high-performance DMEM formulation features 4.5 g/L glucose and L-glutamine to support rapidly proliferating and energy-intensive cell types. The absence of sodium pyruvate and HEPES makes this basal medium ideal for researchers requiring precise control over their culture conditions.

Our high-performance DMEM formulation features 4.5 g/L glucose and L-glutamine to support rapidly proliferating and energy-intensive cell types. The absence of sodium pyruvate and HEPES makes this basal medium ideal for researchers requiring precise control over their culture conditions.

DMEM (1X), High Glucose with L-Glutamine
(Without Sodium Pyruvate, Without HEPES)

Essential Culture Medium for Demanding Applications

This standard DMEM formulation combines 4.5 g/L glucose with L-glutamine to support robust cell growth, while omitting sodium pyruvate and HEPES for researchers needing controlled metabolic conditions.

Key Characteristics

▸ CO₂ Requirement: Requires 5-10% CO₂ environment to maintain optimal pH (~7.4)
▸ Energy Supply: High glucose concentration fuels rapidly dividing cells
▸ Growth Support: L-glutamine included for protein synthesis (note stability considerations)

Recommended Cell Types

• Fibroblasts and primary cells
• Neuronal cultures
• Cancer cell lines (HeLa, HEK293, etc.)
• Stem cell maintenance

Supplementation Guidelines

Standard additions before use:
✓ 5-10% FBS (for essential growth factors)
✓ 1% penicillin-streptomycin (antibiotic protection)
✓ Fresh L-glutamine (recommended for extended cultures)

Critical Handling Notes

⚠ Glutamine Stability: Degrades at 37°C - refresh weekly or use GlutaMAX™ alternative
⚠ pH Maintenance: CO₂ incubation essential (no HEPES buffering)
⚠ Storage Conditions:

  • 2-8°C protected from light

  • Stable for 12 months unopened

  • Use opened media within 1 month

Common Applications

→ Routine cell culture maintenance
→ Transfection and viral production
→ Protein expression studies
→ Cell proliferation assays