DMEM/F-12 Ham 1:1 Mixture 1X, With L-glutamine, With HEPES, Without Sodium Bicarbonate (1x500ml)

$24.00

Our DMEM/F-12 1:1 mixture combines the nutritional richness of DMEM with the specialized components of Ham's F-12 to create an optimal environment for demanding cell culture applications. This complete formulation includes L-glutamine for protein synthesis and HEPES buffer for superior pH stability, while excluding sodium bicarbonate for specialized experimental setups.

Our DMEM/F-12 1:1 mixture combines the nutritional richness of DMEM with the specialized components of Ham's F-12 to create an optimal environment for demanding cell culture applications. This complete formulation includes L-glutamine for protein synthesis and HEPES buffer for superior pH stability, while excluding sodium bicarbonate for specialized experimental setups.

DMEM/F-12 Ham (1:1 Mixture) with L-Glutamine & HEPES

Premium Hybrid Medium for Demanding Cell Culture Applications

This advanced 1:1 blend of DMEM and Ham's F-12 creates an optimal nutritional environment for sensitive and fastidious cell types. The formulation combines:
✓ DMEM's high nutrient density - Ideal for rapidly proliferating cells
✓ Ham's F-12 specialized components - Supports more fastidious cell requirements
✓ HEPES buffering - Maintains pH stability in variable CO₂ conditions
✓ L-Glutamine - Essential for protein synthesis and cell metabolism

Critical Handling Note

⚠ Aseptic Technique Required - Always handle under sterile conditions to prevent microbial contamination and preserve culture integrity

Optimal For

• Primary cell cultures (epithelial, neuronal, endothelial)
• Stem cell applications (iPSCs, organoids, 3D cultures)
• Specialized research (toxicology, drug screening, cancer studies)
• Hybridoma technology

Recommended Supplementation

Customize based on cell type requirements:

  • 5-15% FBS (or serum-free alternatives)

  • 1% antibiotics (penicillin-streptomycin)

  • Specialized growth factors (EGF, FGF, etc.)

  • Additional L-glutamine for extended cultures

Key Advantages

✔ Superior nutritional profile supports challenging cell types
✔ HEPES buffer minimizes pH fluctuations
✔ Versatile for both adherent and suspension cultures
✔ Excellent baseline for serum-free formulations

Storage & Stability

  • Temperature: 2-8°C (do not freeze)

  • Light Protection: Required (amber bottle or foil-wrapped)

  • Shelf Life: 12 months unopened

  • Opened Stability: Use within 4 weeks with proper aseptic technique

*Note: For bicarbonate-dependent systems, supplement with 1.2-3.7 g/L sodium bicarbonate as required by your experimental setup.*