Human Kidney CAF

$2,743.77

Human Kidney Cancer-Associated Fibroblasts (CAFs) are primary cells isolated from human kidney tumor specimens and carefully cultured to maintain their native tumor microenvironment properties. The cells are expanded in T75 tissue culture flasks pre-coated with AlphaBioCoat solution for 30 minutes to enhance cellular attachment and subsequently maintained in specialized CAF Growth Medium for 3-7 days to ensure optimal proliferation. For distribution, first-passage cells are harvested and cryopreserved at high viability in freezing medium, with each vial containing a minimum concentration of 1×10⁶ cells/mL. These ready-to-use cryovials provide researchers with biologically relevant CAFs that retain their functional characteristics, making them ideal for studying kidney cancer stroma interactions, tumor progression mechanisms, and therapeutic development applications while ensuring consistent, reliable performance in experimental settings.

Human Kidney Cancer-Associated Fibroblasts (CAFs) are primary cells isolated from human kidney tumor specimens and carefully cultured to maintain their native tumor microenvironment properties. The cells are expanded in T75 tissue culture flasks pre-coated with AlphaBioCoat solution for 30 minutes to enhance cellular attachment and subsequently maintained in specialized CAF Growth Medium for 3-7 days to ensure optimal proliferation. For distribution, first-passage cells are harvested and cryopreserved at high viability in freezing medium, with each vial containing a minimum concentration of 1×10⁶ cells/mL. These ready-to-use cryovials provide researchers with biologically relevant CAFs that retain their functional characteristics, making them ideal for studying kidney cancer stroma interactions, tumor progression mechanisms, and therapeutic development applications while ensuring consistent, reliable performance in experimental settings.

Human Kidney Cancer-Associated Fibroblasts (CAFs) Product Specifications

Quality Control & Characterization
All Human Kidney CAF cultures undergo comprehensive validation to ensure research reliability. The cells test negative for endothelial (von Willebrand Factor/Factor VIII), epithelial (cytokeratin 18), and smooth muscle (α-SMA) markers, confirming fibroblast purity. Microbiological testing verifies absence of bacteria, yeast, fungi, and mycoplasma contamination. Positive expression of characteristic CAF markers (FAP, PDGFR, Vimentin, PDPN, and CD70) is confirmed through immunophenotyping. Cells maintain stable growth characteristics for 3-5 passages when subcultured at recommended 1:2 to 1:3 ratios.

Research Applications
These primary kidney CAFs are suitable for:
• Tumor microenvironment interaction studies
• Cell adhesion and migration assays
• Molecular analysis (PCR, Western blot, immunoprecipitation)
• Cellular characterization (immunofluorescence, flow cytometry)
• Development of advanced 3D tumor models
• Drug screening and therapeutic development

Cell Handling Protocol

Shipping & Storage

  • Delivered in cryovials on dry ice

  • For optimal results:

    • Immediate culture initiation recommended

    • Alternative storage: liquid nitrogen vapor phase

    • Avoid -70°C storage (reduces cell viability)

Thawing & Culture Initiation

  1. Rapidly thaw cells in 37°C water bath (≤2 minutes)

  2. Surface sterilize vial with 70% ethanol

  3. Remove cryoprotectant via centrifugation (125 × g, 5-10 min)

  4. Resuspend in fresh CAF Growth Medium

  5. Plate in AlphaBioCoat-treated T75 flask (6-8 mL coating solution, 15 min)

  6. Maintain at 37°C with 5% CO₂ (pre-equilibrate medium 15 min)

Subculture Procedure

  1. Remove spent medium and rinse with PBS

  2. Add 2-3 mL Cell Detachment Solution (5-15 min incubation)

  3. Neutralize with complete medium (6-8 mL)

  4. Replate at recommended split ratio (1:2 to 1:3) in freshly coated flasks

  5. Perform medium changes every 3-4 days

Cryopreservation

  • Use complete growth medium with 5% DMSO

  • Store long-term in liquid nitrogen vapor phase