Human Liver CAF

$2,942.27

Human Liver Cancer-Associated Fibroblasts (CAFs) are primary cells isolated from human liver tumor specimens and carefully cultured to maintain their native tumor microenvironment properties. The cells are expanded in T75 tissue culture flasks pre-coated with AlphaBioCoat solution for 30 minutes to enhance cellular attachment and subsequently maintained in specialized CAF Growth Medium for 3-7 days to ensure optimal proliferation. For distribution, first-passage cells are harvested and cryopreserved at high viability in freezing medium, with each vial containing a minimum concentration of 1×10⁶ cells/mL. These ready-to-use cryovials provide researchers with biologically relevant CAFs that retain their functional characteristics, making them ideal for studying liver cancer stroma interactions, tumor progression mechanisms, and therapeutic development applications while ensuring consistent, reliable performance in experimental settings.

Human Liver Cancer-Associated Fibroblasts (CAFs) are primary cells isolated from human liver tumor specimens and carefully cultured to maintain their native tumor microenvironment properties. The cells are expanded in T75 tissue culture flasks pre-coated with AlphaBioCoat solution for 30 minutes to enhance cellular attachment and subsequently maintained in specialized CAF Growth Medium for 3-7 days to ensure optimal proliferation. For distribution, first-passage cells are harvested and cryopreserved at high viability in freezing medium, with each vial containing a minimum concentration of 1×10⁶ cells/mL. These ready-to-use cryovials provide researchers with biologically relevant CAFs that retain their functional characteristics, making them ideal for studying liver cancer stroma interactions, tumor progression mechanisms, and therapeutic development applications while ensuring consistent, reliable performance in experimental settings.

Human Liver Cancer-Associated Fibroblasts (CAFs) - Product Specifications

Quality Control & Characterization
Our Human Liver CAFs undergo rigorous quality testing to ensure research reliability. The cells demonstrate negative expression of endothelial (von Willebrand Factor/Factor VIII), epithelial (cytokeratin 18), and smooth muscle (α-SMA) markers, confirming fibroblast purity. All cultures test negative for microbial contamination (bacteria, yeast, fungi, and mycoplasma). The cells positively express characteristic CAF markers including FAP, PDGFR, Vimentin, PDPN, and CD70, with stable growth maintained for 3-5 passages at recommended 1:2 to 1:3 split ratios.

Research Applications
These primary liver CAFs are ideal for:
• Tumor microenvironment studies
• Cell-cell interaction and adhesion assays
• Molecular analysis (PCR, Western blot, immunoprecipitation)
• Cellular characterization (immunofluorescence, flow cytometry)
• Development of advanced liver cancer models
• Drug screening and therapeutic development

Cell Handling Protocol

Shipping & Storage

  • Shipped frozen on dry ice

  • Optimal storage:

    • Immediate culture recommended

    • Alternative: liquid nitrogen vapor phase

    • Avoid -70°C storage (reduces viability)

Thawing & Culture Initiation

  1. Rapid thaw in 37°C water bath (≤2 minutes)

  2. Surface sterilize with 70% ethanol

  3. Centrifuge (125 × g, 5-10 min) to remove cryoprotectant

  4. Resuspend in fresh CAF Growth Medium

  5. Plate in AlphaBioCoat-treated T75 flask (6-8 mL coating, 15 min)

  6. Incubate at 37°C, 5% CO₂ (pre-equilibrate medium 15 min)

Subculture Procedure

  1. Remove medium and rinse with PBS

  2. Add 2-3 mL Cell Detachment Solution (5-15 min)

  3. Neutralize with complete medium (6-8 mL)

  4. Replate at 1:2 to 1:3 ratio in freshly coated flasks

  5. Change medium every 3-4 days

Cryopreservation

  • Use complete growth medium with 5% DMSO

  • Store long-term in liquid nitrogen vapor phase