Immortalized Brain Vascular Fibroblast Growth Medium

$600.00

Optimized specifically for the culture and expansion of immortalized human brain vascular fibroblasts, our Immortalized Brain Vascular Fibroblast Growth Medium (IBVF-GM) ensures superior cell viability, proliferation, and phenotypic stability. This complete, ready-to-use medium is formulated with essential growth factors, vitamins, and supplements to maintain robust fibroblast growth while preserving their unique vascular and extracellular matrix (ECM) production properties.

Optimized specifically for the culture and expansion of immortalized human brain vascular fibroblasts, our Immortalized Brain Vascular Fibroblast Growth Medium (IBVF-GM) ensures superior cell viability, proliferation, and phenotypic stability. This complete, ready-to-use medium is formulated with essential growth factors, vitamins, and supplements to maintain robust fibroblast growth while preserving their unique vascular and extracellular matrix (ECM) production properties.

Product Specifications

Format: Frozen cryovials or Ready-to-Use Liquid
Volume: 500mL (Custom volumes available)
Special Formulations:

  • Neurovascular Niche Medium (with astrocyte-conditioned factors)

  • Hypoxia-Adapted Medium (1% O₂ for ischemic modeling)

Product Overview

A premium, scientifically optimized medium designed specifically for culturing immortalized brain vascular fibroblasts (hBVFs, HBVP lines). This formulation supports robust proliferation while maintaining BBB-relevant functionality, including extracellular matrix (ECM) secretion and angiogenic potential—critical for neurovascular research.

Physicochemical Properties

  • pH: 7.2–7.4 (stable CO₂ buffering)

  • Osmolality: 310–330 mOsm/kg (physiologically balanced)

  • Color: Red (phenol red for pH monitoring)

Key Benefits

✔ Validated Performance:

  • Consistent doubling time (30–36 hrs) for hBVFs

  • Preserves cerebrovascular markers (CD13+, PDGFR-β+, α-SMA+)

  • Supports ECM production (Collagen IV, laminin by ELISA/ICC)

✔ Functional Assay-Ready:

  • Ideal for angiogenesis studies (tube formation, endothelial co-culture)

  • Compatible with hypoxia experiments (optional 1% O₂ formulation)

✔ Quality Guaranteed:

  • Pre-screened for mycoplasma, viruses, and sterility

  • Available in serum-free or low-serum options

Storage & Stability

  • Liquid Medium:

    • Unopened: 12 months at 2–8°C

    • Opened: 4 weeks at 2–8°C

  • Frozen Medium:

    • –20°C (avoid repeated freeze-thaws)

Recommended Culture Protocol

Subculture Steps:

  1. Rinse cells with Ca²⁺/Mg²⁺-free PBS.

  2. Detach using 0.25% trypsin-EDTA (2–5 min, 37°C).

  3. Neutralize with trypsin inhibitor, centrifuge (200 ×g, 5 min).

  4. Reseed at 5,000–10,000 cells/cm² in coated flasks (T25/T75).

Feeding Schedule:

  • Refresh medium every 2–3 days

  • Passage at 70–80% confluency (prevents senescence)