Immortalized Fibroblast Cryopreservation Medium

$600.00

Our Immortalized Fibroblast Cryopreservation Medium is a ready-to-use, serum-free formulation specifically optimized for freezing and recovering immortalized fibroblast lines. This advanced medium ensures >90% post-thaw viability while maintaining cellular phenotype and functionality across multiple freeze-thaw cycles.

Our Immortalized Fibroblast Cryopreservation Medium is a ready-to-use, serum-free formulation specifically optimized for freezing and recovering immortalized fibroblast lines. This advanced medium ensures >90% post-thaw viability while maintaining cellular phenotype and functionality across multiple freeze-thaw cycles.

Product Format

  • State: Frozen

  • Volume: 20 mL

Custom Options

  • DMSO-free (ideal for sensitive applications)

  • Serum-supplemented (optimized for primary fibroblasts)

Product Description

A specialized serum-free cryopreservation medium designed to maximize post-thaw recovery of immortalized fibroblast lines (e.g., NIH/3T3, MRC-5, hTERT-BJ1). This ready-to-use formulation supports ≥95% post-thaw viability, preserving cellular morphology, proliferation potential, and biomarker expression.

Key Features

✔ ≥95% post-thaw viability across multiple fibroblast types
✔ Xeno-free and animal component–free
✔ No preparation required — ready to use
✔ Validated for:
 • Skin fibroblasts (e.g., BJ-5ta)
 • Lung fibroblasts (e.g., MRC-5)
 • Cancer-associated fibroblasts (e.g., CAFs)
✔ Compatible with:
 • Programmable controlled-rate freezers
 • Passive cooling devices (e.g., Mr. Frosty™)
 • Liquid nitrogen vapor phase storage

Physicochemical Properties

  • pH: 7.2–7.4

  • Osmolality: 1000–1200 mOsm/kg

  • Sterility: 0.22 µm filtered

Recommended Protocol

A. Freezing Procedure

  1. Harvest cells at 80–90% confluency using 0.25% Trypsin-EDTA.

  2. Neutralize trypsin with growth medium and pellet at 200 × g for 5 min.

  3. Resuspend cells at 2–5 × 10⁶ cells/mL in growth medium.

  4. Mix 1:1 with pre-chilled cryopreservation medium (final: 1–2.5 × 10⁶ cells/mL).

  5. Aliquot 1 mL per cryovial.

  6. Freeze using one of the following:
     • Controlled-rate freezer: −1°C/min to −80°C → transfer to liquid nitrogen
     • Passive cooling (Mr. Frosty™): −80°C overnight → transfer to liquid nitrogen

B. Thawing Procedure

  1. Thaw vial quickly in a 37°C water bath (≤90 seconds).

  2. Dilute thawed cells 1:10 in pre-warmed growth medium.

  3. Centrifuge at 200 × g for 5 min to remove cryoprotectants.

  4. Resuspend and seed at 2× the normal density for recovery.