Immortalized Pancreatic Fibroblast Growth Medium

$600.00

Our Immortalized Pancreatic Fibroblast Growth Medium (IPF-GM) is a complete, ready-to-use formulation specifically optimized to support the unique metabolic requirements of pancreatic fibroblasts. This advanced medium promotes authentic phenotype maintenance while enabling robust proliferation for reliable studies of pancreatic fibrosis, tumor microenvironments, and stromal-epithelial interactions.

Our Immortalized Pancreatic Fibroblast Growth Medium (IPF-GM) is a complete, ready-to-use formulation specifically optimized to support the unique metabolic requirements of pancreatic fibroblasts. This advanced medium promotes authentic phenotype maintenance while enabling robust proliferation for reliable studies of pancreatic fibrosis, tumor microenvironments, and stromal-epithelial interactions.

Product Format

  • State: Frozen or Liquid

  • Volume: 500 mL

Note: Custom formulations available upon request (e.g., serum-free, hypoxia-adapted).

Product Description

A complete, serum-enriched culture medium formulated specifically for the expansion and maintenance of immortalized human pancreatic fibroblasts (e.g., hPSC-EC-FBs, PSC-HPFs). Designed to support robust growth, viability, and the retention of functional fibroblast phenotypes, including α-SMA, FAP, and PDGFR-β expression, as well as extracellular matrix (ECM) secretion and cytokine production.

Physicochemical Properties

  • pH: 7.2–7.4

  • Osmolality: 290–310 mOsm/kg

  • Color Indicator: Red (contains phenol red)

Key Features

✔ Optimized for pancreatic fibroblast cell lines (e.g., hPSC-EC-FBs)
✔ Doubling time: ~28–32 hours
✔ Maintains activated fibroblast phenotype (α-SMA⁺, FAP⁺, PDGFR-β⁺)
✔ Promotes ECM protein deposition (e.g., collagen I, fibronectin)
✔ Sterility-tested and free from mycoplasma and viral contaminants
✔ Available in low-serum or serum-free formats

Storage & Handling

  • Liquid Storage: 2–8°C

  • Frozen Storage: –20°C (for long-term)

  • Shelf Life:
     • Unopened: 12 months at 2–8°C
     • After opening: use within 4 weeks

  • Avoid repeated freeze-thaw cycles

Recommended Culture Protocol

Subculture Procedure

  1. Rinse cells with PBS (Ca²⁺/Mg²⁺-free).

  2. Detach cells using an appropriate cell detachment solution.

  3. Neutralize enzymatic activity with a neutralizing solution.

  4. Centrifuge at 200 × g for 5 min and resuspend in fresh culture medium.

  5. Seed at 5,000–10,000 cells/cm² in T25 or T75 flasks coated as needed.

Feeding Schedule

  • Replace medium every 48–72 hours.

  • Passage at 70–80% confluency to avoid overgrowth and phenotypic drift.