Immortalized Prostate Fibroblast Growth Medium

$600.00

This complete, serum-supplemented growth medium is specifically formulated for the culture and expansion of immortalized human prostate fibroblasts (e.g., WPMY-1, PSC27). Designed to support robust cell proliferation while preserving critical fibroblast morphology and biomarker expression, this medium ensures high viability, stable phenotype, and consistent performance in both standard and advanced prostate research applications.

This complete, serum-supplemented growth medium is specifically formulated for the culture and expansion of immortalized human prostate fibroblasts (e.g., WPMY-1, PSC27). Designed to support robust cell proliferation while preserving critical fibroblast morphology and biomarker expression, this medium ensures high viability, stable phenotype, and consistent performance in both standard and advanced prostate research applications.

Product Format: Frozen or Liquid
Volume: 500 mL
Custom Options:

  • Serum-free formulation (with human platelet lysate)

  • CAF-conditioned medium (for tumor microenvironment studies)

Description:

This complete, optimized culture medium is formulated for the growth and expansion of immortalized human prostate fibroblasts (e.g., WPMY-1, BHPrS1). Engineered to promote consistent proliferation, this medium preserves fibroblast morphology and key biomarker expression, including FSP-1, α-SMA, and vimentin. Ideal for modeling prostate stroma, CAF-related studies, and co-culture systems involving epithelial or cancer cells.

Key Features:

✔ Validated for prostate fibroblast lines (e.g., WPMY-1 doubling time: 24–30 hours)
✔ Maintains stromal markers: Vimentin+ (>95%), FSP-1+ (>90%), α-SMA+ (variable)
✔ Promotes extracellular matrix (ECM) deposition: collagen I/III, fibronectin
✔ Pre-screened for mycoplasma, viral contamination, and sterility
✔ Compatible with 2D and 3D culture systems (e.g., Matrigel, collagen scaffolds)

Physicochemical Properties:

  • pH: 7.2–7.4

  • Osmolality: 300–320 mOsm/kg

  • Color: Red (contains phenol red pH indicator)

Storage & Stability:

  • Liquid form: Store at 2–8°C

  • Frozen form: Store at –20°C for long-term storage

  • Shelf Life:
     • Unopened: 12 months at 2–8°C
     • After opening: 4 weeks at 2–8°C

  • Avoid multiple freeze-thaw cycles

Recommended Culture Protocol:

Subculturing:

  1. Rinse cells with Ca²⁺/Mg²⁺-free PBS

  2. Detach using a suitable cell detachment solution

  3. Neutralize with neutralization buffer or growth medium

  4. Centrifuge at 200 ×g for 5 minutes

  5. Resuspend in fresh growth medium and seed at 5,000–10,000 cells/cm² in T25 or T75 flasks

Feeding Schedule:

  • Replace medium every 48–72 hours

  • Passage at 70–80% confluency (avoid overgrowth)