Human Lung CAF Squamous Cell Carcinoma

$2,644.21

Human Lung Cancer-Associated Fibroblasts (CAFs) are primary cells isolated from human lung tumor specimens and cultured under optimized conditions to maintain their tumor microenvironment characteristics. The cells are expanded in T75 flasks pre-coated with AlphaBioCoat solution for 30 minutes and maintained in specialized CAF Growth Medium for 3-7 days to ensure proper attachment and proliferation. For distribution, first-passage cells are harvested and cryopreserved at high viability in freezing medium, with each vial containing a minimum of 1×10⁶ cells/mL. These ready-to-use cryovials preserve the native biological properties of lung CAFs, making them ideal for studying tumor-stroma interactions, extracellular matrix remodeling, and other cancer biology applications in pulmonary malignancies.

Human Lung Cancer-Associated Fibroblasts (CAFs) are primary cells isolated from human lung tumor specimens and cultured under optimized conditions to maintain their tumor microenvironment characteristics. The cells are expanded in T75 flasks pre-coated with AlphaBioCoat solution for 30 minutes and maintained in specialized CAF Growth Medium for 3-7 days to ensure proper attachment and proliferation. For distribution, first-passage cells are harvested and cryopreserved at high viability in freezing medium, with each vial containing a minimum of 1×10⁶ cells/mL. These ready-to-use cryovials preserve the native biological properties of lung CAFs, making them ideal for studying tumor-stroma interactions, extracellular matrix remodeling, and other cancer biology applications in pulmonary malignancies.

Human Lung Cancer-Associated Fibroblasts (CAFs) Product Specifications

Quality Control & Characterization
Our Human Lung CAFs undergo rigorous testing to ensure research-grade quality. The cells demonstrate negative expression of endothelial (von Willebrand Factor/Factor VIII), epithelial (cytokeratin 18), and smooth muscle (α-SMA) markers, confirming fibroblast purity. All cultures are certified free from microbial contamination (bacteria, yeast, fungi, and mycoplasma). The cells positively express characteristic CAF markers including FAP, PDGFR, Vimentin, PDPN, and CD70, and maintain stable growth for 3-5 passages when subcultured at a 1:2 to 1:3 ratio.

Research Applications
These primary lung CAFs are suitable for:
• Tumor microenvironment interaction studies
• Cell adhesion and migration assays
• Molecular analysis (PCR, Western blot, immunoprecipitation)
• Cellular characterization (immunofluorescence, flow cytometry)
• Development of advanced cancer models

Cell Handling Protocol

Shipping & Storage

  • Delivered frozen on dry ice

  • For optimal results:

    • Immediate culture initiation recommended

    • Alternative storage: liquid nitrogen vapor phase

    • Avoid -70°C storage (compromises cell viability)

Thawing & Culture Initiation

  1. Rapidly thaw cells in 37°C water bath (2 minutes maximum)

  2. Sterilize vial exterior with 70% ethanol

  3. Remove cryoprotectant via centrifugation (125 × g, 5-10 min)

  4. Resuspend in fresh CAF Growth Medium

  5. Plate in AlphaBioCoat-precoated T75 flask (6-8 mL coating solution, 15 min)

  6. Maintain at 37°C with 5% CO₂ (pre-equilibrate medium 15 min before use)

Subculture Procedure

  1. Aspirate spent medium and rinse with PBS

  2. Add 2-3 mL Cell Detachment Solution (5-15 min incubation)

  3. Neutralize with complete medium (6-8 mL)

  4. Replate at recommended split ratio (1:2 to 1:3) in freshly coated flasks

  5. Perform medium changes every 3-4 days

Cryopreservation

  • Use complete growth medium with 5% DMSO

  • Store long-term in liquid nitrogen vapor phase