Human Lung CAF Carcinoma

$2,790.00

Our Human Lung Cancer-Associated Fibroblasts (CAF) are isolated directly from human lung tumor tissue to ensure biological relevance and high performance in cancer research applications. The cells are initially cultured in T75 tissue culture flasks pre-coated with AlphaBioCoat™ for 30 minutes to promote optimal adhesion and viability. They are then incubated in specialized CAF Growth Medium for 3–7 days to support healthy cell expansion.

Our Human Lung Cancer-Associated Fibroblasts (CAF) are isolated directly from human lung tumor tissue to ensure biological relevance and high performance in cancer research applications. The cells are initially cultured in T75 tissue culture flasks pre-coated with AlphaBioCoat™ for 30 minutes to promote optimal adhesion and viability. They are then incubated in specialized CAF Growth Medium for 3–7 days to support healthy cell expansion.

Product Testing & Quality Assurance

Our Human Lung Cancer-Associated Fibroblasts (CAF) are rigorously tested to ensure purity, functionality, and reliability in research applications. Each cell lot is:

  • Negative for von Willebrand Factor (Factor VIII), Cytokeratin 18, and α-Smooth Muscle Actin, confirming the absence of endothelial and epithelial contaminants.

  • Free from bacteria, yeast, fungi, and mycoplasma.

  • Positive for key CAF markers including FAP, PDGFR, Vimentin, PDPN, and CD70, validating their fibroblastic and tumor-associated phenotype.

Cells can be expanded for 3–5 passages at a recommended split ratio of 1:2 or 1:3.

Applications

Human Lung CAFs are ideal for a wide range of biomedical and cancer research applications, including:

  • Cell-cell interaction assays

  • Adhesion and migration studies

  • PCR and Western blotting

  • Immunoprecipitation

  • Flow cytometry and immunofluorescence

  • Generation of derivative cell lines for advanced modeling

Shipping & Handling

Format: Frozen Vials on Dry Ice
To preserve maximum cell viability, we recommend immediate thawing and culturing upon receipt. If storage is necessary, vials must be kept in the vapor phase of liquid nitrogen. Do not store at -70°C, as this will compromise cell viability.

Thawing Protocol:

  1. Thaw vial quickly in a 37°C water bath (~2 minutes), keeping the cap above water to avoid contamination.

  2. Once thawed, disinfect the vial with 70% ethanol and proceed under sterile conditions.

  3. Centrifuge cells at 125 x g for 5–10 minutes, discard supernatant, and resuspend pellet in fresh growth medium.

  4. Coat T-Flask with AlphaBioCoat (6–8 mL for 15 min), rinse with 1X PBS, and discard rinse.

  5. Pre-incubate growth medium in the flask for 15 minutes to stabilize pH (7.0–7.6).

  6. Transfer cells into the prepared flask and incubate at 37°C in 5% CO₂.

Subculturing Instructions

Recommended Culture Vessel: T-75 flask
Note: Do not shake or hit flasks to dislodge cells to avoid clumping.

  1. Remove and discard old medium. Rinse with 1X PBS.

  2. Add 2–3 mL of Cell Detachment Solution and observe under a microscope until detachment (5–15 minutes).

  3. Coat a new flask with AlphaBioCoat (6–8 mL for 15 minutes), rinse with 1X PBS, and discard.

  4. Add complete growth medium, gently pipette to suspend cells.

  5. Transfer appropriate aliquots to new flasks.

  6. Incubate at 37°C.

  7. Recommended Split Ratio: 1:2 to 1:3

  8. Medium Renewal: Every 3–4 days

Cryopreservation Reagents

Use Complete Growth Medium + 5% DMSO for cryopreservation.