MEM 1X with Earle's, L-Glutamine, NEAA

$16.00

This Minimum Essential Medium formulation combines Earle's balanced salt solution with supplemental L-glutamine and NEAA to create an optimal growth environment for a wide range of mammalian cell types. The carefully balanced composition supports cell viability and proliferation while maintaining physiological conditions.

Quantity:

MEM 1X with Earle's Salts, L-Glutamine & NEAA

Essential Cell Culture Medium for Mammalian Cells

This enhanced MEM formulation combines Earle's balanced salts with L-glutamine and non-essential amino acids (NEAA) to create a nutritionally complete medium for optimal cell growth. The carefully balanced composition provides:
✓ Essential nutrients for cell viability and proliferation
✓ Improved performance through NEAA supplementation
✓ Stable physiological conditions with Earle's salts

Key Applications

• Primary cell culture (fibroblasts, epithelial cells)
• Cell line maintenance (HeLa, Vero, MDCK)
• Biopharmaceutical production (vaccines, monoclonal antibodies)
• Research applications (cytotoxicity testing, protein expression studies)

Critical Components

✔ Earle's Salts - Maintains proper osmotic balance
✔ L-Glutamine (2-4mM) - Essential for protein synthesis
✔ NEAA - Reduces metabolic burden on cultured cells

Storage & Handling

Complete medium: Store at 2-8°C; use within 4 weeks
L-Glutamine: Store frozen (-5°C to -20°C); add fresh before use
Protect from light to maintain medium integrity

Supplementation Guidelines

Recommended additions:
✓ 5-10% FBS for most applications
✓ 1% antibiotics for contamination control
✓ Fresh L-glutamine for extended culture periods

Note: For serum-free applications, additional growth factors may be required.