Human Prostate Cancer-Associated Fibroblasts (CAFs) - Product Specifications
Quality Control & Validation
All Human Prostate CAF cultures undergo comprehensive characterization to ensure research-grade quality. Testing confirms negative expression of endothelial (von Willebrand Factor/Factor VIII), epithelial (cytokeratin 18), and smooth muscle (α-SMA) markers, verifying fibroblast purity. Microbiological testing demonstrates absence of bacteria, yeast, fungi, and mycoplasma contamination. Cells positively express established CAF markers including FAP, PDGFR, Vimentin, PDPN, and CD70, with stable growth maintained for 3-5 passages at recommended 1:2 to 1:3 split ratios.
Research Applications
These primary prostate CAFs are suitable for:
• Tumor microenvironment interaction studies
• Cell adhesion and migration assays
• Molecular analysis (PCR, Western blot, immunoprecipitation)
• Cellular characterization (immunofluorescence, flow cytometry)
• Development of advanced 3D tumor models
• Drug screening and therapeutic development
Cell Handling Protocol
Shipping & Storage
Delivered in cryovials on dry ice
Optimal storage conditions:
Immediate culture initiation recommended
Alternative storage: liquid nitrogen vapor phase
Avoid -70°C storage (compromises cell viability)
Thawing & Culture Initiation
Rapidly thaw cells in 37°C water bath (≤2 minutes)
Surface sterilize vial with 70% ethanol
Remove cryoprotectant via centrifugation (125 × g, 5-10 min)
Resuspend in fresh CAF Growth Medium
Plate in AlphaBioCoat-treated T75 flask (6-8 mL coating solution, 15 min)
Maintain at 37°C with 5% CO₂ (pre-equilibrate medium 15 min)
Subculture Procedure
Remove spent medium and rinse with PBS
Add 2-3 mL Cell Detachment Solution (5-15 min incubation)
Neutralize with complete medium (6-8 mL)
Replate at recommended split ratio (1:2 to 1:3) in freshly coated flasks
Perform medium changes every 3-4 days
Cryopreservation
Use complete growth medium with 5% DMSO
Store long-term in liquid nitrogen vapor phase