Prostate CAF

$3,000.00

Human Prostate Cancer-Associated Fibroblasts (CAFs) are primary cells isolated from human prostate tumor tissue and cultured under optimized conditions to maintain their tumor microenvironment characteristics. The cells are expanded in T75 tissue culture flasks pre-coated with AlphaBioCoat solution for 30 minutes to enhance cellular attachment and subsequently maintained in specialized CAF Growth Medium for 3-7 days to ensure proper proliferation. For distribution, first-passage cells are harvested and cryopreserved at high viability in freezing medium, with each vial containing a minimum concentration of 1×10⁶ cells/mL. These ready-to-use cryovials preserve the native biological properties of prostate CAFs, making them ideal for studying tumor-stroma interactions, extracellular matrix remodeling, and other cancer biology applications in prostate malignancies while ensuring consistency and reliability for research purposes.

Human Prostate Cancer-Associated Fibroblasts (CAFs) are primary cells isolated from human prostate tumor tissue and cultured under optimized conditions to maintain their tumor microenvironment characteristics. The cells are expanded in T75 tissue culture flasks pre-coated with AlphaBioCoat solution for 30 minutes to enhance cellular attachment and subsequently maintained in specialized CAF Growth Medium for 3-7 days to ensure proper proliferation. For distribution, first-passage cells are harvested and cryopreserved at high viability in freezing medium, with each vial containing a minimum concentration of 1×10⁶ cells/mL. These ready-to-use cryovials preserve the native biological properties of prostate CAFs, making them ideal for studying tumor-stroma interactions, extracellular matrix remodeling, and other cancer biology applications in prostate malignancies while ensuring consistency and reliability for research purposes.

Human Prostate Cancer-Associated Fibroblasts (CAFs) - Product Specifications

Quality Control & Validation
All Human Prostate CAF cultures undergo comprehensive characterization to ensure research-grade quality. Testing confirms negative expression of endothelial (von Willebrand Factor/Factor VIII), epithelial (cytokeratin 18), and smooth muscle (α-SMA) markers, verifying fibroblast purity. Microbiological testing demonstrates absence of bacteria, yeast, fungi, and mycoplasma contamination. Cells positively express established CAF markers including FAP, PDGFR, Vimentin, PDPN, and CD70, with stable growth maintained for 3-5 passages at recommended 1:2 to 1:3 split ratios.

Research Applications
These primary prostate CAFs are suitable for:
• Tumor microenvironment interaction studies
• Cell adhesion and migration assays
• Molecular analysis (PCR, Western blot, immunoprecipitation)
• Cellular characterization (immunofluorescence, flow cytometry)
• Development of advanced 3D tumor models
• Drug screening and therapeutic development

Cell Handling Protocol

Shipping & Storage

  • Delivered in cryovials on dry ice

  • Optimal storage conditions:

    • Immediate culture initiation recommended

    • Alternative storage: liquid nitrogen vapor phase

    • Avoid -70°C storage (compromises cell viability)

Thawing & Culture Initiation

  1. Rapidly thaw cells in 37°C water bath (≤2 minutes)

  2. Surface sterilize vial with 70% ethanol

  3. Remove cryoprotectant via centrifugation (125 × g, 5-10 min)

  4. Resuspend in fresh CAF Growth Medium

  5. Plate in AlphaBioCoat-treated T75 flask (6-8 mL coating solution, 15 min)

  6. Maintain at 37°C with 5% CO₂ (pre-equilibrate medium 15 min)

Subculture Procedure

  1. Remove spent medium and rinse with PBS

  2. Add 2-3 mL Cell Detachment Solution (5-15 min incubation)

  3. Neutralize with complete medium (6-8 mL)

  4. Replate at recommended split ratio (1:2 to 1:3) in freshly coated flasks

  5. Perform medium changes every 3-4 days

Cryopreservation

  • Use complete growth medium with 5% DMSO

  • Store long-term in liquid nitrogen vapor phase