Human Ovarian CAF

$3,023.04

Human Ovarian Cancer-Associated Fibroblasts (CAFs) are primary cells isolated from ovarian tumor specimens and carefully cultured to preserve their native tumor microenvironment properties. The cells are expanded in T75 tissue culture flasks pre-coated with AlphaBioCoat solution for 30 minutes to enhance cellular attachment and subsequently maintained in specialized CAF Growth Medium for 3-7 days to ensure optimal proliferation. For distribution, first-passage cells are harvested and cryopreserved at high viability in freezing medium, with each vial containing a minimum concentration of 1×10⁶ cells/mL. These ready-to-use cryovials provide researchers with biologically relevant CAFs that retain their functional characteristics, making them ideal for studying ovarian cancer stroma interactions, tumor progression mechanisms, and therapeutic development applications while ensuring consistent, reliable performance in experimental settings.

Human Ovarian Cancer-Associated Fibroblasts (CAFs) are primary cells isolated from ovarian tumor specimens and carefully cultured to preserve their native tumor microenvironment properties. The cells are expanded in T75 tissue culture flasks pre-coated with AlphaBioCoat solution for 30 minutes to enhance cellular attachment and subsequently maintained in specialized CAF Growth Medium for 3-7 days to ensure optimal proliferation. For distribution, first-passage cells are harvested and cryopreserved at high viability in freezing medium, with each vial containing a minimum concentration of 1×10⁶ cells/mL. These ready-to-use cryovials provide researchers with biologically relevant CAFs that retain their functional characteristics, making them ideal for studying ovarian cancer stroma interactions, tumor progression mechanisms, and therapeutic development applications while ensuring consistent, reliable performance in experimental settings.

Human Ovarian Cancer-Associated Fibroblasts (CAFs) - Product Specifications

Quality Assurance & Characterization
Our Human Ovarian CAFs undergo rigorous quality control to ensure research reliability. The cells test negative for endothelial (von Willebrand Factor/Factor VIII), epithelial (cytokeratin 18), and smooth muscle (α-SMA) markers, confirming fibroblast purity. All cultures are certified free from microbial contamination (bacteria, yeast, fungi, and mycoplasma). The cells demonstrate positive expression of established CAF markers including FAP, PDGFR, Vimentin, PDPN, and CD70, maintaining stable growth characteristics for 3-5 passages when subcultured at recommended 1:2 to 1:3 ratios.

Research Applications
These primary ovarian CAFs are ideal for:
• Tumor microenvironment interaction studies
• Cell adhesion and invasion assays
• Molecular analysis (PCR, Western blot, immunoprecipitation)
• Cellular characterization (immunofluorescence, flow cytometry)
• Development of advanced 3D tumor models
• Drug screening and therapeutic development

Cell Handling Protocol

Shipping & Storage

  • Delivered in cryovials on dry ice

  • Optimal storage conditions:

    • Immediate culture initiation recommended

    • Alternative storage: liquid nitrogen vapor phase

    • Avoid -70°C storage (reduces cell viability)

Thawing & Culture Initiation

  1. Rapidly thaw cells in 37°C water bath (≤2 minutes)

  2. Surface sterilize vial with 70% ethanol

  3. Remove cryoprotectant via centrifugation (125 × g, 5-10 min)

  4. Resuspend in fresh CAF Growth Medium

  5. Plate in AlphaBioCoat-treated T75 flask (6-8 mL coating solution, 15 min)

  6. Maintain at 37°C with 5% CO₂ (pre-equilibrate medium 15 min)

Subculture Procedure

  1. Remove spent medium and rinse with PBS

  2. Add 2-3 mL Cell Detachment Solution (5-15 min incubation)

  3. Neutralize with complete medium (6-8 mL)

  4. Replate at 1:2 to 1:3 ratio in freshly coated flasks

  5. Perform medium changes every 3-4 days

Cryopreservation

  • Use complete growth medium with 5% DMSO (Catalog #CGM5)

  • Store long-term in liquid nitrogen vapor phase