Human Ovarian CAF Metastatic

$2,750.21

Human Ovarian Cancer-Associated Fibroblasts (CAFs) are primary cells isolated from ovarian tumor specimens and carefully cultured to preserve their native tumor microenvironment characteristics. The cells are expanded in T75 flasks pre-treated with AlphaBioCoat solution for 30 minutes to enhance attachment, then maintained in specialized CAF Growth Medium for 3-7 days to ensure optimal proliferation. For distribution, first-passage cells are harvested and cryopreserved at high viability, with each vial containing a minimum concentration of 1×10⁶ cells/mL in freezing medium. These ready-to-use cryovials provide researchers with biologically relevant CAFs that retain their functional properties, making them ideal for studying ovarian cancer stroma interactions, tumor progression mechanisms, and developing novel therapeutic approaches.

Human Ovarian Cancer-Associated Fibroblasts (CAFs) are primary cells isolated from ovarian tumor specimens and carefully cultured to preserve their native tumor microenvironment characteristics. The cells are expanded in T75 flasks pre-treated with AlphaBioCoat solution for 30 minutes to enhance attachment, then maintained in specialized CAF Growth Medium for 3-7 days to ensure optimal proliferation. For distribution, first-passage cells are harvested and cryopreserved at high viability, with each vial containing a minimum concentration of 1×10⁶ cells/mL in freezing medium. These ready-to-use cryovials provide researchers with biologically relevant CAFs that retain their functional properties, making them ideal for studying ovarian cancer stroma interactions, tumor progression mechanisms, and developing novel therapeutic approaches.

Human Ovarian Cancer-Associated Fibroblasts (CAFs) - Product Specifications

Quality Assurance & Characterization
Our Human Ovarian CAFs undergo comprehensive validation to ensure research reliability. The cells test negative for endothelial (von Willebrand Factor/Factor VIII), epithelial (cytokeratin 18), and smooth muscle (α-SMA) markers, confirming fibroblast purity. All cultures are certified free from microbial contamination (bacteria, yeast, fungi, and mycoplasma). The cells demonstrate positive expression of established CAF markers including FAP, PDGFR, Vimentin, PDPN, and CD70, maintaining stable growth for 3-5 passages when subcultured at a recommended 1:2 to 1:3 ratio.

Research Applications
These primary ovarian CAFs are ideal for:
• Tumor microenvironment interaction studies
• Cell adhesion and invasion assays
• Molecular profiling (PCR, Western blot, immunoprecipitation)
• Cellular characterization (immunofluorescence, flow cytometry)
• Development of advanced 3D tumor models

Cell Handling Protocol

Shipping & Storage

  • Delivered in cryovials on dry ice

  • Optimal storage conditions:

    • Immediate culture initiation recommended

    • Alternative: liquid nitrogen vapor phase

    • Avoid -70°C storage (reduces cell viability)

Thawing & Culture Initiation

  1. Rapid thaw in 37°C water bath (≤2 minutes)

  2. Surface sterilize with 70% ethanol

  3. Remove cryoprotectant via centrifugation (125 × g, 5-10 min)

  4. Resuspend in fresh CAF Growth Medium

  5. Plate in AlphaBioCoat-treated T75 flask (6-8 mL coating solution, 15 min)

  6. Maintain at 37°C with 5% CO₂ (pre-equilibrate medium 15 min)

Subculture Procedure

  1. Remove spent medium and rinse with PBS

  2. Add 2-3 mL Cell Detachment Solution (5-15 min incubation)

  3. Neutralize with complete medium (6-8 mL)

  4. Replate at 1:2 to 1:3 ratio in freshly coated flasks

  5. Perform medium changes every 3-4 days

Cryopreservation

  • Use complete growth medium with 5% DMSO

  • Store long-term in liquid nitrogen vapor phase