Bronchial CAF

$2,895.00

Human Bronchial Cancer-Associated Fibroblasts (CAFs) are isolated from primary bronchial tumor tissue and cultured in T75 flasks pre-coated with AlphaBioCoat solution for 30 minutes to enhance cell adhesion. The cells are maintained in specialized CAF Growth Medium for 3-7 days until optimal expansion is achieved. For distribution, first-passage cells are harvested and cryopreserved at a minimum concentration of 1×10⁶ cells per milliliter in freezing medium to ensure maximum viability upon thawing. These primary cells retain their characteristic CAF phenotype and are suitable for various cancer microenvironment studies, providing researchers with a biologically relevant model system for investigating tumor-stroma interactions in bronchial carcinomas. The cryopreserved vials are shipped ready for immediate culture initiation in standard laboratory conditions

Human Bronchial Cancer-Associated Fibroblasts (CAFs) are isolated from primary bronchial tumor tissue and cultured in T75 flasks pre-coated with AlphaBioCoat solution for 30 minutes to enhance cell adhesion. The cells are maintained in specialized CAF Growth Medium for 3-7 days until optimal expansion is achieved. For distribution, first-passage cells are harvested and cryopreserved at a minimum concentration of 1×10⁶ cells per milliliter in freezing medium to ensure maximum viability upon thawing. These primary cells retain their characteristic CAF phenotype and are suitable for various cancer microenvironment studies, providing researchers with a biologically relevant model system for investigating tumor-stroma interactions in bronchial carcinomas. The cryopreserved vials are shipped ready for immediate culture initiation in standard laboratory conditions

Quality-Controlled Human Bronchial CAFs for Cancer Research

Cell Authentication & Safety Testing
Our Human Bronchial Cancer-Associated Fibroblasts (CAFs) undergo comprehensive quality control to ensure research reliability. The cells test negative for endothelial (von Willebrand Factor/Factor VIII), epithelial (cytokeratin 18), and smooth muscle (α-SMA) contamination, confirming fibroblast purity. All cultures are certified free from bacteria, yeast, fungi, and mycoplasma contamination. These CAFs maintain optimal growth characteristics for 3-5 passages when subcultured at a 1:2 to 1:3 split ratio.

Research Applications
These primary bronchial CAFs are ideal for:

  • Tumor microenvironment interaction studies

  • Cell adhesion and migration assays

  • Molecular biology techniques (PCR, Western blot, IP)

  • Cellular characterization (immunofluorescence, flow cytometry)

  • Development of specialized cancer models

Cell Handling Protocol

Shipping & Storage

  • Shipped frozen on dry ice

  • For best results:

    • Immediate culture initiation recommended

    • Alternative storage: liquid nitrogen vapor phase

    • Avoid -70°C storage (reduces viability)

Thawing & Culture Initiation

  1. Rapidly thaw cells in 37°C water bath (~2 min)

  2. Decontaminate vial with 70% ethanol

  3. Remove cryoprotectant by centrifugation (125 × g, 5-10 min)

  4. Resuspend in fresh CAF Growth Medium

  5. Plate in AlphaBioCoat-coated T75 flask (6-8 mL, 15 min coating)

  6. Maintain at 37°C, 5% CO₂ (pre-equilibrate medium 15 min)

Subculture Procedure

  1. Remove spent medium and rinse with PBS

  2. Add 2-3 mL Cell Detachment Solution (5-15 min, 37°C if needed)

  3. Neutralize with complete medium (6-8 mL)

  4. Replate at 1:2 to 1:3 ratio in freshly coated flasks

  5. Refresh medium every 3-4 days

Cryopreservation

  • Use complete growth medium with 5% DMSO

  • Store in liquid nitrogen vapor phase for long-term preservation